skip to Main Content
Back To Top
- What is the ideal cell number for analysis? The ideal cell number varies and depends on the experimental goals. We typically input 5000 to 7000 cells.
- What is the capture rate? Cell capture rate depends on several variables, including input cell number and viability. A typical experiment ranges from 30-60%. Please see 10X Genomics website for details.
- How should the cells be prepared for 10X? It is recommended that investigators should optimize their cell isolation procedure prior to 10X experiments. In general, we recommend cell viability of >80% for optimal cell input. The Human Immunology Center Flow Cytometry Sorting Core can be used to isolate viable cells or there are protocols available for cell isolation by MACs beads.
- How many samples should I run? The number of samples should be determined based on your experimental goals. We can support up to 8 samples each day, however it is highly recommended that the investigator perform a pilot experiment before committing to large scale experiment.