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Single Cell Genomics Core Pipeline

  • CELL INPUT/EXPERIMENTAL DESIGN – Core personnel will conduct an initial consultation to discuss sample specific details of your study and to help begin planning the timeline of the experiment

  • CELL SORTING – In partnership with the Human Immunology Center, cells can be sorted to isolate viable populations free of debris to be run through the 10X controller for best results

  • 10X GENOMICS – Purified samples are run through 10X controller and individually bar-coded libraries are generated and pooled

  • SEQUENCING – In partnership with the DFCI MBCF, pooled library samples are sequenced

  • BIOINFORMATIC ANALYSIS – Raw data reads are processed using robust software methods and strict QC to generate browseable Loupe Cell data files.

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